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Zyagen provides research scientists with
access to high quality ready to use total protein of most rat, mouse, dog,
rabbit, human, rabbit, guinea pig, bovine, hamster, equine, sheep, pig, and
chicken tissues that might difficult to obtain because of size, anatomical complexity,
or high protease activity.
Tissues are freshly dissected and homogenized
in modified RIPA buffer supplemented with a cocktail of 7 protease inhibitors to
minimize proteolysis.
Protein is
stable for one year if stored properly at -70oC.
The
quality of protein as indicated by the absence of smear (no degradation) and
sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with
Coomassie blue staining. The integrity of protein is tested by
immunoblotting using specific beta-actin antibody.
The extracted protein is provided in RIPA
buffer at a concentration of 5mg/ml and ready for immediate
use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing
gels and SDS-capillary electrophoresis. Protein can also be provided in
denatured SDS Sample buffer for only Western blotting use.
Custom Services: If you are interested
in tissue lysate of rat, mouse or any other species to be specifically
prepared to meet your application, our custom service scientists can make it for
you. Contact customer service for more information.
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