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Life Science

Products

Ready-to-use Tissue Sections

Ready-to-use Tissue Microrrays

Ready-to-use  Total RNA    

Ready-to-use Tissue Lysates

Ready-to-use cDNA

Ready-to-use Genomic DNA

Ready-to-use Set of  RNA, DNA, & Protein from the same tissue

Premade Northern Blots

Premade Western Blots

Biochemicals, Kits and Reagents

Immunohistochemistry Dectection Kits
RNA Isolation Kits
Buffers and Solutions

Custom Research Services

Immunohistochemistry Custom Services

Tissue Sectioning

Custom Services

Northern blot Analysis Custom Services

Western blot Analysis

Custom Services

Rat and Mouse Embryo, Paraffin and frozen sections

Zyagen offers high quality paraffin and frozen sagittal and coronal  sections of mouse and rat whole embryo at different stages of pregnancy from  E10 to E17 for mouse and E12 to E19 for rat. The pregnancy is precisely determined by observation of vaginal plug. Plug date is considered  day one of gestation. The sagittal sections are made close to the midline of each embryo and most of major embryonic tissues are shown in each section.

Zyagen also offers paraffin coronal sections shown many embryonic tissues such as eye, heart, pituitary, tooth, ovary, testis, limbs, and neuronal tubes at different stages of pregnancy. These tissue sections are ideal for studying developmental changes in protein and mRNA expression in each embryonic tissue during pregnancy by immunohistochemistry and in situ hybridization, respectively.  Contact customer service for the availability of other developmental prenatal tissue sections not listed below. If you are also interested in tissue sections of any mouse or rat postnatal tissues, we can make it for you.

 

Mouse Whole Embryo sagittal sections

Rat Whole Embryo Sagittal Sections

Mouse Whole Embryo coronal sections

Paraffin Sections: Pregnancy-staged embryos are harvested from pregnant animals, fixed in 10% neutral buffered formalin or cold 4% paraformaldehyde in PBS (upon the client request) for 5-24 hr (depending on the embryo size), washed,  dehydrated in serial ethanol solutions, cleaned with xyelene, and embedded in paraffin. Embryos are cut at a thickness of 7-10 microns and sections are mounted on slides treated for adherence. Each slide contains 2-4 sections depending on the section size. Slides are shipped at room temperature in tightly closed slide boxes or slide mailers.

Frozen Sections: Pregnancy-staged embryos are  freshly harvested and snapped frozen in O.C.T. medium for cryosectioning. Frozen embryos are cut at a thickness of 7-10 microns and sections are mounted on slides treated for adherence.  Each slide contains 2 sections. Slides are shipped on dry ice in tightly closed slide boxes or slide mailers.

Applications: Paraffin and frozen tissue sections are prepared in RNase-, DNase-free environment and are ideal for cellular localization of specific protein and mRNA in embryonic tissues during pregnancy by immunohistochemistry and in situ hybridization, respectively.

Custom Services: If you are interested in embryo sections that are not listed below or required tissue sections to be specifically prepared to meet your application, our tissue sectioning custom service scientists can make it for you. We also offer tissue sections of  any prenatal and postnatal rat and mouse tissue for developmental studies. Contact customer service for more information.

 

Mouse Whole Embryo,  Paraffin and Frozen  Sagittal Sections  
Stage of Pregnancy Paraffin Frozen Quantity Price
Day 10 of pregnancy MP-104-10 MF-104-10 10 slides $212
Day 11 of pregnancy MP-104-11 MF-104-11 10 slides $212
Day 12 of pregnancy MP-104-12 MF-104-12 10 slides $212
Day 13 of pregnancy MP-104-13 MF-104-13 10 slides $212
Day 14 of pregnancy MP-104-14 MF-104-14 10 slides $212
Day 15 of pregnancy MP-104-15 MF-104-15 10 slides $212
Day 16 of pregnancy MP-104-16 MF-104-16 10 slides $212
Day 17 of pregnancy MP-104-17 MF-104-17 10 slides $212
Set E10-E17 of pregnancy, 4 slides each MP-104-008 MF-104-008 8X4 slides $612

 
Rat Whole Embryo,  Paraffin and Frozen  Sagittal Sections  
Stage of Pregnancy Paraffin Frozen Quantity Price
Day 12 of pregnancy RP-104-12 RF-104-12 10 slides $212
Day 13 of pregnancy RP-104-13 RF-104-13 10 slides $212
Day 14 of pregnancy RP-104-14 RF-104-14 10 slides $212
Day 15 of pregnancy RP-104-15 RF-104-15 10 slides $212
Day 16 of pregnancy RP-104-16 RF-104-16 10 slides $212
Day 17 of pregnancy RP-104-17 RF-104-17 10 slides $212
Day 18 of pregnancy RP-104-18 RF-104-18 10 slides $212
Day 19 of pregnancy RP-104-19 RF-104-19 10 slides $212
Set 12-19 of pregnancy, 4 slides each RP-104-008 RF-104-008 8X4 slides $612

 

Mouse Whole Embryo,  Paraffin Coronal Sections shown different embryonic tissues

at different stages of pregnancy 
Stage of Pregnancy

Tissues 

Quantity  Price
Brain Eye Heart Pituitary Testis Ovary Tooth Limbs Neural Tube
Day 10 of pregnancy MP-201-10 MP-106-10 MP-801-10 MP-502-10       MP-113-10 MP-238-10 10 Slides $290
Day 11 of pregnancy MP-201-11 MP-106-11 MP-801-11 MP-502-11       MP-113-11 MP-238-11 10 Slides $290
Day 12 of pregnancy MP-201-12 MP-106-12 MP-801-12 MP-502-12       MP-113-12 MP-238-12 10 Slides $290
Day 13 of pregnancy MP-201-13 MP-106-13 MP-801-13 MP-502-13 MP-401-13 MP-502-13 MP-112-13 MP-113-13 MP-238-13 10 Slides $290
Day 14 of pregnancy MP-201-14 MP-106-14 MP-801-14 MP-502-14 MP-401-14 MP-502-14 MP-112-14 MP-113-14 MP-238-14 10 Slides $290
Day 15 of pregnancy MP-201-15 MP-106-15 MP-801-15 MP-502-15 MP-401-15 MP-502-15 MP-112-15 MP-113-15 MP-238-15 10 Slides $290
Day 16 of pregnancy MP-201-16 MP-106-16 MP-801-16 MP-502-16 MP-401-16 MP-502-16 MP-112-16 MP-113-16 MP-238-16 10 Slides $290
Day 17 of pregnancy MP-201-17 MP-106-17 MP-801-17 MP-502-17 MP-401-17 MP-502-17 MP-112-17 MP-113-17 MP-238-17 10 Slides $290
Day 18 of pregnancy MP-201-18 MP-106-18 MP-801-18 MP-502-18 MP-401-18 MP-502-18 MP-112-18 MP-113-18 MP-238-18 10 Slides $290
Set of any 5 stages, 4 slides each MP-201-005 MP-106-005 MP-801-005 MP-502-005

MP-401-005

MP-502-005

MP-112-005

MP-113-005 MP-238-005 9x4Slides $612
Set of 9 stages, 4 slides each MP-201-009 MP-106-009 MP-801-009 MP-502-009       MP-113-009