Welcome To Zyagen Laboratories

Search

Ordering Information

Life Science

Products

Biochemicals, Kits and Reagents

Custom Research Services

Coronal. Sagittal, and Horizontal Paraffin Sections: Brains are carefully removed from the skulls, fixed for 16-24 hr in 10% neutral buffered formalin or 4% paraformeldehyde (upon the client request), and embedded in paraffin. Paraffin blocks are sectioned at a thickness of 5-7 microns in coronal , sagittal, or horizontal orientation,  and mounted on slides treated for adherence. Each slide contains 2-4 sections. Paraffin sections are shipped at room temperature in tightly closed slide boxes or slide mailers.

Coronal. Sagittal, and Horizontal Frozen Sections: Brains are snapped frozen in O.C.T. medium and sectioned at a thickness of 7-10 microns in coronal, sagittal, or horizontal orientation,  and mounted on slides treated for adherence. Each slide contains 2 sections. Frozen sections are shipped in tightly closed slide boxes or slide mailers on dry ice. Fixed and cryoprotected in sucrose rat and mouse brain sections are also available upon request.

Coronal. Sagittal, and Horizontal Floating Frozen Sections. The floating sections are specifically made for Floating Immunostaining in culture dishs. Brains are fixed O/N in 10% buffered formalin, cryoprotected for 24-36hr, snapped frozen in O.C.T. medium,  and sectioned at a thickness of 30 microns in coronal, sagittal, or horizontal orientation. The frozen thick sections are shipped in 6- or 12--well culture plates (one section per each well) on dry ice. Upon arrival, keep culture plates at -80oC and never allow them to warm-up to the room temperature (sections will attach to the culture plates at RT). Before use, place culture plate on dry ice and gently add 2 ml PBS to each well to allow sections floating in the buffer. Remove plate from dry ice and follow your protocol of immunostaining at room temperature.  Restore the unused sections in the culture plates at -80oC. Sections are good for several months from the date of shipping if stored properly.

Floating brain sections from paraformeldehyde- or buffered formalin-perifused animals in different thickness and orientations are also available upon request. Contact us for more information.

Rat and mouse strain: Brain tissue sections are routinely prepared from Sprague Dawley rats and  CD1 mice.  Contact us for the availability of brain tissue sections of other strains.

Applications: Paraffin and frozen brain tissue sections are prepared in RNase-, DNase-, and protease-free environment and are ideal for  immunohistochemistry and in situ hybridization.

Custom Research Services: If you are interested in paraffin or frozen brain sections of any rat or mouse strain (inbred, outbred, mutant, hybrid, transgenic) in any orientation, our scientists can make it for you. We can also process brain sections in your specifications to fit your need (fixation, section thickness, slide coating, ...etc)  Click here for more information.

Ordering Information: Mouse Brain Coronal Tissue Sections, Paraffin and Frozen  

(click on brain region number to display an illustration) 

Ordering Information: Rat Brain Coronal Tissue Sections, Paraffin and Frozen  

(click on brain region number to display an illustration)