Premade Tissue Western Blots

Zyagen offers the largest selection of Western blots of rat, mouse, human, dog, hamster, rabbit, guinea pig, bovine, sheep, pig, equine, chicken, and monkey tissues to fit the specific need of scientists from different research fields. Blots are prepared from high quality tissue total protein extracted blotted onto PVDF  membranes.

Blots can be used to:

• Quickly assess specific protein expression across different tissues.

• Determine size and relative abundance of protein  in different tissues.

• In addition to the quick assessment of protein expression, the multiple-species Western blots are ideal for testing cross-reactivity of polyclonal and monoclonal antibodies across species.

Benefits:

• Ready-to-use Western blots from high quality tissue lysates that are difficult  to obtain because of size, high-protease tissue content, or anatomical complexity of the tissues which requires knowledge, experience, and technical skill to dissect the areas of interest freely of any surrounding tissues.

• Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, protein extraction, quantification, gel electrophoresis, and blotting).

• High standards of quality control

• Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to labor and cost of laboratory materials, supplies and  animals (purchasing, transportation, housing, disposable of carcasses and waste materials).

• Reusable for at least two times, just strip and use with other antibody.

Quality control:

Every step of preparation of blots, from harvesting tissues and extraction of protein to the blotting, is carefully monitored to ensure the superior quality and performance.

• Blots are made from high quality tissue lysates that is homogenized in RIPA buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.

• The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.

• The integrity of the blotted protein is tested by immunostaining of b-actin.

Description:

The total protein (75mg each) samples are freshly extracted from tissues, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes.  Each blot contains 10 to 22 lanes in addition to the prestained protein molecular weight standards. Each blot is shipped in a sealed bag at room temperature and is good for one year  if stored dry at 4^oC in a sealed bag with descant.

Custom Services: Zyagen also offers custom Western blotting services to make blots of  the client specifications. Click here for more information.

Mouse Tissue Premade Western Blots:

• Mouse Adult Tissue Western blots

• Mouse Pregnancy-Staged Embryo, Placenta, and Mammary Gland Western blots

• Mouse Developmental Tissue Western Blots

Rat Tissue Premade Western Blots:

• Rat Adult Tissue Western Blots

• Rat Pregnancy-staged Embryo, Placenta, and Mammary Gland Western Blots

• Rat Developmental Tissue Western Blots

Human Tissue Premade Western Blots

Dog Tissue Premade Western Blots

Cat Tissue Premade Western Blots

Rabbit Tissue Premade Western Blots

Hamster Tissue premade Western Blots

Guinea Pig Tissue Premade Western Blots

Bovine Tissue Premade Western Blots

Sheep Tissue Premade Western Blots

Pig Tissue Premade Western Blots

Equine Tissue Premade Western Blots

Chicken Tissue Premade Western Blots

Monkey Tissue Premade Western Blots

Cross-species Tissue Premade Western Blots

Western Blot Analysis Kits

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