Zyagen offers the largest selection of
Western blots of rat, mouse, human, dog, hamster, rabbit, guinea pig, bovine,
sheep, pig, equine, chicken, and monkey tissues to fit the
specific need of scientists from different research fields. Blots are prepared
from high quality tissue total protein extracted blotted onto PVDF membranes.
Blots can be used to:
Quickly assess specific protein expression across different tissues.
Determine size
and relative abundance of protein in different tissues.
In addition to the quick assessment of
protein expression, the multiple-species Western blots are ideal for testing
cross-reactivity of polyclonal and monoclonal antibodies across species.
Benefits:
Ready-to-use Western blots from high quality
tissue lysatesthat are difficult to obtain
because of size, high-protease tissue content, or anatomical complexity of
the tissues which
requires knowledge, experience, and technical skill to dissect the areas
of interest freely of any surrounding tissues.
Saving labor by eliminating tedious and
time-consuming laboratory procedures (harvesting and homogenization of tissues,
protein extraction, quantification, gel electrophoresis, and blotting).
High standards of quality control
Economic, using multiple tissues at a cost
much lower than it would take to do in your laboratory. Think to labor and
cost of laboratory materials, supplies and animals (purchasing,
transportation, housing, disposable of carcasses and waste materials).
Reusable for at least two times, just strip and
use with other antibody.
Quality control:
Every step of preparation of blots, from
harvesting tissues and extraction of protein to the blotting, is carefully
monitored to ensure the superior quality and performance.
Blots are made from high quality tissue lysates that is homogenized in RIPA
buffer containing cocktail
of 7 mammalian protease inhibitors. The quality of protein as indicated by the
absence of smear (no degradation) and sharpness and resolution of protein bands
are
verified by denatured SDS-PAGE with Coomassie blue staining.
The efficiency of transfer
is routinely checked by staining protein on membranes with reversible Ponceau staining.
The integrity of the
blotted protein is tested by immunostaining of b-actin.
Description:
The total protein (75mg
each) samples are freshly extracted from
tissues, boiled in
SDS sample buffer, fractionated though
12.5% SDS-denaturing polyacralamide gel eletrophoresis (SDS PAGE),
and
blotted onto PVDF membranes.
Each blot contains 10 to 22 lanes in addition to the prestained protein
molecular weight standards. Each blot is shipped in a sealed bag at room
temperature and is good for one year if stored dry at 4oC in a sealed bag
with descant.
Custom Services: Zyagen also offers custom Western blotting services to make blots
of the client specifications. Click here
for more information.
