Zyagen offers highly optimized peroxidase immunohistochemistry kits to detect cell surface and intracellular antigens in frozen or paraffin-embedded tissue sections mounted on slides, floating sections in culture plates, and immobilized cells with high sensitivity and low background. Chromogen DAB (3, 3’-diaminobenzidine) is used as a substrate of horseradish peroxidase (HRP) for visualization of antigenic structures in the tissues.
Each kit contains all reagents needed for immunostaining including serum blocking buffer, peroxidase suppresser, antibody diluent, biotinylated secondary antibody (cross-absorbed and purified), streptavidin-peroxidase conjugate, DAB substrate, hematoxylin, and mounting medium.
Kits are easy to use, provided with instructions for each step from preparation of tissue sections to mounting slides with coverslips. In addition to the immunostaining procedure, the user manual also contains detailed technical information about processing frozen and paraffin tissue sections for immunostaining, antigen retrieval, and trouble-shootings.
- High specificity and sensitivity
- Low background
- Standardization with high reproducible results
- Low cost
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