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RNA Products
Tissue Total RNA Products
Universal Reference RNA Products
Match Set of Tissue RNA, DNA, & Protein Products
Zyagen offers high quality intact total RNA, DNA, and total protein simultaneously isolated of the same tissue of C57BL/6 mouse or Sprague Dawley rat. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction. All RNA samples are treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact 28S and 18S ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). It is provided in RNase-free water, 1mM sodium citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml. DNA samples are suitable for PCR, Southern blot analysis, and DNA library construction. Samples are treated with DNase-free RNase to remove contaminant RNA and provided in 1.5-ml tubes at a concentration of 1mg/ml in TE buffer. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody. Protein samples are…
Genomic DNA Products
Aquatic Animal Genomic DNA
Control Genomic DNA
Zyagen offers a pure and intact high molecular size control genomic DNA of more than 40 different species (male and female). Genomic DNA is extracted from whole blood or tissues of single donor and treated with DNase-free RNase to remove contaminant RNA. Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluoreometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. Genomic DNA samples of multiple donors of many species are also available upon request. Applications: PCR Amplification Southern blot analysi Genomic DNA methylation Single-nucleotide polymorphism (SNP) Analysis Profiling studies in gene expression DNA library construction Quality Control: The integrity and purity of genomic DNA was tested by agarose gel electrophoresis and spectrophotometer Ratio of A260/280 is >1.8 Free of RNA contamination Successful PCR amplification of Beta-Actin Successful digestion of DNA by EcoR1 and/or Hind III enzyme.
Plant Genomic DNA
Zyagen offers a pure and intact high molecular size control genomic DNA of 32 different plant species. Genomic DNA is extracted from plant leaves and subjected for treatment to remove contaminant RNA, protein and polysaccharide, Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluorometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. Applications: PCR Amplification Southern blot analysi Genomic DNA methylation Single-nucleotide polymorphism (SNP) Analysis Profiling studies in gene expression DNA library construction Quality Control: The integrity and purity of genomic DNA was tested by agarose gel electrophoresis and spectrophotometer Ratio of A260/280 is >1.8 Free of RNA, protein, and polysaccharides contamination Successful PCR amplification of house-keeping genes Successful digestion of DNA by EcoR1 and/or Hind III enzyme.
Human & Animal Tissue Genomic DNA
Zyagen offers a pure and intact high molecular size genomic DNA extracted from large variety of tissues of human and many animal species. Genomic DNA is extracted from single tissue of single donor and treated with DNase-free RNase to remove contaminant RNA. Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluoreometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. Applications: PCR Amplification Southern blot analysi Genomic DNA methylation Single-nucleotide polymorphism (SNP) Analysis Profiling studies in gene expression DNA library construction Quality Control: The integrity and purity of genomic DNA was tested by agarose gel electrophoresis and spectrophotometer Ratio of A260/280 is >1.8 Free of RNA contamination Successful PCR amplification of Beta-Actin Successful digestion of DNA by EcoR1 and/or Hind III enzyme.
Tissue Lysates Products
Tissue Lysates Products
Total Protein of different Tissues of Different Species. Click on Ecah Image for Enlargment Zyagen provides high quality ready to use Total Protein of wide variety of rat, mouse, cat, dog, rabbit, human, rabbit, guinea pig, bovine, hamster, equine, sheep, pig, chicken, monkey, and plant tissues that might difficult to obtain because of size, anatomical complexity, or high protease activity. Tissues are freshly dissected and homogenized in modified modified protein lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis. Protein is stable for one year if stored properly at -70oC. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody. The extracted protein is provided in vials at a concentration of 5mg/ml and ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction. Protein can also be provided in denatured SDS…
Cell Compartment Protein Products
cDNA Products
Tissue cDNA Products
[gallery link="file" ids="20326,20327,20328"] Gel images of PCR amplification of cDNA samples of different tissues of different species using Beta-Actin Primers. NC: Negative Control; cDNA was made without RT enzyme. Click on Each Image for Enlargement Zyagen offers the largest selection of ready to use full length tissue cDNA. The PCR-ready to use first strand cDNA is synthesized from high quality RNA isolated from a wide variety of rat, mouse, cat, dog, human, monkey, rabbit, guinea pig, hamster, pig, minipig, bovine, sheep, chicken, equine, and plant tissues. The cDNA is primed with oligo dT or random hexame primers and is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, and gene cloning and target sequencing. As quality control test, each cDNA sample is tested by PCR amplification of beta-actin. Each product is shipped in 1.5 ml tube and is enough for 30 PCR reactions.
Universal Reference cDNA
Tissue Sections
Frozen Tissue Sections
Paraffin Tissue Sections
Microarray Tissue Sections
Western & Northern Blot Products
Premade Tissue Western Blots
Ready -to-use Western blots made of bovine, cat, chicken, dog, equine, Guinea pig, hamster, human, minipig, monkey, mouse (CD1 and C57BL/6), pig, rabbit, rat, sheep, and plant tissues
Premade Tissue Northern Blots
Readay -to-use Northern blots made of bovine, cat, chicken, dog, equine, Guinea pig, hamster, human, minipig, monkey, mouse (CD1 and C57BL/6), pig, rabbit, rat, sheep, and plant tissues
Antibodies
Zyagen offer large selections of monoclonal and polyclonal antibodies raised in different host species including rabbit, mouse, goat, and chicken. All antibodies are stringently validated for different applications including western blot (immunoblot), immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), flow cytometry, radioimmunoassay, neutralization, immunoprecipitation (IP) and ELISA. All antibodies have been immunoaffinity purified and rigorously tested which enables Zyagen to provide the highest quality products available. We also offer many positive and negative controls such as frozen/paraffin tissue sections and tissue lysates of multiple species to be used in conjunction with each antibody. Please contact Zyagen technical support for the availability of these products. Also, be sure to check back frequently as we are constantly adding new products. [wcmg_alphabetical_search]
Antibodies A-Z
Zyagen offer large selections of monoclonal and polyclonal antibodies raised in different host species including rabbit, mouse, goat, and chicken. All antibodies are stringently validated for different applications including western blot (immunoblot), immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), flow cytometry, radioimmunoassay, neutralization, immunoprecipitation (IP) and ELISA. All antibodies have been immunoaffinity purified and rigorously tested which enables Zyagen to provide the highest quality products available. We also offer many positive and negative controls such as frozen/paraffin tissue sections and tissue lysates of multiple species to be used in conjunction with each antibody. Please contact Zyagen technical support for the availability of these products. Also, be sure to check back frequently as we are constantly adding new products. [wcmg_alphabetical_search]
Kits, Reagents & Biochemical
RNA Isolation Kits
Zyagen total RNA isolation Kit provides a rapid method for the isolation and purification of total RNA from a wide range of sources, including animal cells and tissues, plant tissues and bacteria. RNA extraction is based on disruption of cells in guanidinium thiocyanate detergent solutions, followed by organic extraction and alcohol precipitation of the RNA. All reagents needed for isolatation, analysis, and storage of RNA are included, making kit of an excellent value. The kit isolate all sizes of RNA, from large mRNA and ribosomal RNA, microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA may be used for analysis by Northern blot, RNase protection, primer extension, RT-PCR/real-time PCR, in vitro translation, and for construction of cDNA libraries. The kit contains reagents sufficient for the isolation of RNA from 10 g tissue or approximately 109 cells in culture. FEATURES AND BENEFITS Isolated RNA is of the highest quality and integrity. All RNA species can be isolated, including large mRNA, ribosomal RNA, microRNA (miRNA), and small interfering RNA (siRNA). Total RNA is isolated from high RNA content tissues such as liver and spleen, fatty tissues such as brain, and fibrous tissues such as heart and muscle. Purified RNA can be used in a number of downstream applications including real-time PCR, reverse…
Genomic DNA isolation Kit
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Protein Isolation Kits
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Buffers and Reagents
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Northern Blot Analysis Kits
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Western Blot Analysis Kits
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SERVICES
Histology Services
Western Blot Analysis Services
Immunohistochemistry Services
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