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Human Tissue Lysates


Total Protein of different Tissues of Different Species. Click on Each Image for Enlargement

Zyagen offers high quality ready to use total protein of human adult tissues which are obtained from certified tissue banks in USA. Tissues are freshly harvested by surgeons during surgical operation or from donors whom have donated their tissues for scientific research. Tissue specimens are procured under strict approved protocols with informed consent and the utmost attention to issues of patient safety, anonymity and confidentiality.

Tissues are freshly dissected and homogenized in protein lysis buffer supplemented with a cocktail of 7 mamalian protease inhibitors to minimize proteolysis. The extracted proteins are precisely quantified, and packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if stored properly at -80oC.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.

The extracted protein is provided in vials at a concentration of 5mg/ml and ready for immediate use in the Lysis Buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins. Proteins extracted can be readily used in many applications, including reporter assays, protein assays, immunoassays, SDS-PAGE, protein purification, and many other applications. Protein can also be provided in denatured SDS Sample buffer for only Western Blotting use.

Custom Services: Zyagen also offers custom services for isolation of total protein of any tissue not listed below or of any other species. Contact Zyagen technical support for more information at zinfo@zyagen.com or at 858 546 0720.

 

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