AATF Antibody: AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases. AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways. Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the beta-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer’s disease (AD), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.
Description
Western blot analysis of AATF in human?Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 ?g/msmall intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 ?g/mL.
Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 ?g/mL. ?
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal AATF Antibody.
Purification
AATF Antibody is immunoaffinity purified IgG.
Host
AATF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
AATF antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.
Application
AATF antibody can be used for the detection of AATF by Western blot at 0.5 – 2 ?g/mL. Antibody can also be used for immunohistochemistry starting at 10 ?g/mL. For immunofluorescence start at 20 ?g/mL.
Tested Application
E, WB, IHC, IF
Buffer
AATF Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3039 – AATF Peptide
Storage
AATF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M
Protein GI Number
7657013
Protein Accession Number
NP_036270
Reference
AATF antibody can be stored at 4˚C, stable for one year. Page G, Lodige I, Kogel D et al. AATF, a novel transcription factor that interacts with Dlk/ZIP kinase and interferes with apoptosis. FEBS Lett. 1999; 462:187-191.
Kawai T, Matsumoto M, Takeda K, et al. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol. Cell Biol. 1998; 18:1642-51.
Lindfors K, Halttunen T, Huotari P, et al. Identification of novel transcription factor-like gene from human intestinal cells. Biochem. Biophys. Res. Commun. 2000; 276:660-6.
Xie J and Guo Q. AATF protects neural cells against oxidative damage induced by amyloid b-peptide. Neurobiol. of Dis. 2004; 16:150-7.]
