Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’.
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Description
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Western blot analysis of acinus in K562 whole cell lysate with acinus antibody at 1 ?g/mL.
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Specifications
Additional Names
Acinus (NP), Acinus (NP), Acinus
Additional Names
Acinus Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
220 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Acinus Antibody.
Purification
Acinus Antibody is affinity chromatography purified via peptide column.
Host
Acinus antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Acinus antibody was raised against a peptide corresponding to amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusS?, or 236 to 251 of human AcinusS, which are identical to those of mouse Acinus. The selected antigenic sequence is located near the N terminus of active peptide p17.
Application
Acinus antibody can be used for detection of acinus by Western blot at 1 ?g/mL. An approximate 220 kDa band can be detected.
Acinus Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2241 – Acinus Peptide
Storage
Acinus antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73. ]
