Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’.
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Description
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (CP) at 1 ?g/mL.
Immunocytochemistry staining of K562 cells using Acinus antibody at 10 ?g/mL.
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Specifications
Additional Names
Acinus (CP), Acinus
Molecular Weight
220 kDa
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Isotype
IgG
Clonality/Clone
This is a polyclonal Acinus Antibody.
Conjugate
None
Additional Names
Acinus Antibody:
Purification
Acinus Antibody is affinity chromatography purified via peptide column.
Host
Acinus antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Acinus antibody was raised against a peptide corresponding to amino acids near the C-terminus of the cleaved active peptide p17, which are identical to those of mouse Acinus.
Application
Acinus antibody can be used for detection of Acinus by Western blot at 1 ?g/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL. For immunofluorescence start at 10 ?g/mL.
Tested Application
E, WB, ICC, IF
Buffer
Acinus Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2215 – Acinus Peptide
Storage
Acinus antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Protein GI Number
5931959
Protein Accession Number
AAD56724
Reference
Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73. ]
