AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.
Description
Western blot analysis of AIF in K562 cell lysate with AIF antibody at 1 ?g/mL.
Immunocytochemistry of AIF in Jurkat cells with AIF antibody at 2 ?g/mL.
Specifications
Additional Names
AIF (NT), AIF
Additional Names
AIF Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
67 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal AIF Antibody.
Purification
AIF Antibody is Ion exchange chromatography purified.
Host
AIF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
AIF antibody was raised against a peptide corresponding to amino acids near the amino terminus of mature human AIF.
Application
AIF antibody can be used for detection of AIF by Western blot at 1 ?g/mL. A 67 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 ?g/mL. For immunofluorescence start at 20 ?g/mL.
AIF Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2239 – AIF Peptide
Storage
AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73. ]
