AIFM3 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist.
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Description
Western blot analysis of NIPSNAP in human brain tissue lysate with NIPSNAP antibody at (A) 0.5 and (B) 1 ?g/mL.
Immunohistochemical staining of human brain tissue using AIFM3 antibody at 2.5 ?g/mL.
Optimal dilutions for each application to be determined by the researcher.
Immunogen
AIFM3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human AIFM3.
Isotype
IgG
Clonality/Clone
This is a polyclonal AIFM3 Antibody.
Conjugate
None
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Purification
AIFM3 Antibody is affinity chromatography purified via peptide column.
Host
AIFM3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
AIFM3 antibody can be used for the detection of AIFM3 by Western blot at 0.5 and 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
AIFM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-4539 – AIFM3 Peptide
Storage
AIFM3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther.2004; 4:139-63
Xie Q, Lin T, Zhang Y, et al. Molecular cloning and characterization of a human AIF-like gene with the ability to induce apoptosis. J. Biol. Chem.2005; 280:19673-81.]
