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ATG16 Antibody

ATG16 Antibody

$315.00

0.1 mg

9999 in stock


Product Description

AUT-4425

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Background

ATG16 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Description
ATG16 Antibody
Western blot analysis of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 1 and (B) 2 ?g/mL.
ATG16 Antibody
Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 ?g/mL.
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Specifications

Additional Names

ATG16 Antibody: Autophagy protein 16, Autophagy related protein 16, ATG16L, APG16

User Note

Optimal dilutions for each application to be determined by the researcher.

Isotype

IgG

Immunogen

ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human ATG16.

Disclaimer

This product is for research use only.

Formulation

Physical State: Liquid?

Concentration: 1 mg/mL

Conjugate

None

Clonality/Clone

This is a polyclonal ATG16 Antibody.

Purification

ATG16 Antibody is affinity chromatography purified via peptide column.

Host

ATG16 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Application

ATG16 antibody can be used for the detection of ATG16 by Western blot at 1 and 2 ?g/mL. Antibody can also be used for immunocytochemistry starting at 2 ?g/mL. For immunofluorescence start at 4 ?g/mL.

Tested Application

E, WB, ICC, IF

Buffer

ATG16 Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat.No. ZP-4425 – ATG16 Peptide

Storage

ATG16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Species Reactivity

H

Protein GI Number

124256480

Protein Accession Number

NP_110430

Reference

  • Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
  • Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
  • Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
  • Mizushima N, Noda T, and Ohsumi Y. Apg16p is required for the function of the apg12p-apg5p conjugate in the yeast autophagy pathway. EMBO J.1999; 18:3888-96.]

AUT-4425?Datasheet

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