CDKN2A Antibody: The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas. CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6. CDKN2A is expressed as at least three distinct isoforms.
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Description
Western blot analysis of CDKN2A in C2C12 cell lysate with CDKN2A antibody at (A) 1 and (B) 2 ?g/mL.
Immunofluorescence of CDKN2A in human colon tissue with CDKN2A antibody at 20 ?g/mL.
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgY
Immunogen
CDKN2A antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human CDKN2A.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal CDKN2A Antibody.
Purification
CDKN2A Antibody is affinity chromatography purified via peptide column.
Host
CDKN2A antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Application
CDKN2A antibody can be used for detection of CDKN2A by Western blot at 1 – 2 ?g/mL. Antibody can also be used for immunoflourescence starting at 20 ?g/mL. For immunofluorescence start at 20 ?g/mL.
CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-4211 – CDKN2A Peptide
Storage
CDKN2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Stone S, Jiang P, Dayananth P, et al. Complex structure and regulation of the p16(MTS1) locus. Cancer Res.1995; 55:2988-94.
Kamb A, Shattuck-Eidens D, Eeles R, et al. Analysis of the p16 gene (CDKN2) as a candidate for the chromosome 9p melanoma susceptibility locus. Nature Genet.1994; 8:22-6.
Kratzke RA, Otterson GA, Lincoln CE, et al. Immunohistochemical analysis of the p16(INK4) cyclin-dependent kinase inhibitor in malignant mesothelioma. J. Nat. Cancer Inst.1995; 87:1870-5.
Stott FJ,; Bates S, James MC, et al. The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2. EMBO J.1998; 17:5001-14.]
