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HINT1 Rabbit Polyclonal Antibody

CAN-4815 Categories: ,
HINT1 Rabbit Polyclonal Antibody

$315.00

0.1 mg

9999 in stock


Product Description

CAN-4815

Background

HINT1 Antibody: Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5′-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.

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Description
HINT1 Antibody
Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ?g/mL.
HINT1 Antibody
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ?g/mL.

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Specifications

Additional Names

HINT1 Antibody: HINT1, Histidine triad nucleotide-binding protein 1, HINT, PKC inhibitory protein, PKCI-1

User Note

Optimal dilutions for each application to be determined by the researcher.

Isotype

IgG

Immunogen

HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1.

Disclaimer

This product is for research use only.

Formulation

Physical State: Liquid?

Concentration: 1 mg/mL

Conjugate

None

Clonality/Clone

This is a polyclonal HINT1 Antibody.

Purification

HINT1 Antibody is affinity chromatography purified via peptide column.

Host

HINT1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Application

HINT1 antibody can be used for detection of HINT1 by Western blot at 1 – 2 ?g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 ?g/mL. For immunofluorescence start at 20 ?g/mL.

Tested Application

E, WB, ICC, IF

Buffer

HINT1 Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat.No. ZP-4815 – HINT1 Peptide

Storage

HINT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Species Reactivity

H, M, R

Protein GI Number

4885413

Protein Accession Number

NP_005331

Reference

  • Lima CD, Klein MG, Weinstein IB, et al. Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins. Proc. Natl. Acad. Sci. USA1996; 93:5357-62.
  • Weiske J and Huber O. Beta-catenin takes a HIT. Cell Cycle2008; 7:1326-31.
  • Wang L, Zhang Y, Li H, et al. Hint1 inhibits growth and activator protein-1 activity in human colon cancer cells. Cancer Res.2007; 67:4700-8.
  • Chen Q, Wang X, O?Neill FA, et al. Is the histidine triad nucleotide-binding protein 1 (HINT1) gene a candidate for schizophrenia? Schizophr. Res.2008; 106:200-7.]

CAN-4815

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