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Human Tissue Custom Made Western Blot-1
Product Description
Human custom made Western blot-1 is your choice of any 10 ?normal healthy human tissues of panel# 1. Total protein will be extracted from the selected tissues and subjected to rigorous quality control procedures before fractionation through large SDS-PAGE.?
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Blots can be used to:
- Quickly assess specific protein expression across different tissues.
- Determine size and relative abundance of protein? in different tissues.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
- Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
- The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
- The integrity of the blotted protein is tested by immunostaining of?b-actin.
Description:
The total protein?samples??(75ug each )?are freshly extracted from?tissues, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE),?and blotted onto PVDF membranes. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4oC in a sealed bag with descant.
