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Human Tissue Premade Western Blot
1 Blot
Product Description
Human tissue Western blots are made of high quality total proteins extracted from freshly harvested 19 different normal healthy tissues (Panel# 1). Protein sample are subjected to rigorous quality control procedures.
Blots can be used to:
Quickly assess specific protein expression across different tissues. Determine size and relative abundance of protein in different tissues.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining. The integrity of the blotted protein is tested by immunostaining of b-actin.
Description:
The total protein samples (75ug each ) are freshly extracted from tissues, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4o C in a sealed bag with descant.
