IRGM Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn’s disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3; this antibody may also recognize these proteins.
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Description
Western blot analysis of IRGM in Rat brain lysate with IRGM antibody at (A) 1 and (B) 2 ?g/mL.
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Specifications
Additional Names
IRGM Antibody: Immunity related GTPase family, IFL1, IRGM1, LRG47, LRG-47
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal IRGM Antibody.
Purification
IRGM Antibody is affinity chromatography purified via peptide column.
Host
IRGM antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
IRGM antibody can be used for detection of IRGM by Western blot at 1 – 2 ?g/mL.
Tested Application
E, WB
Buffer
IRGM Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-4543 – IRGM Peptide
Storage
IRGM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M, R
Protein GI Number
118764009
Protein Accession Number
AAI28169
Reference
Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
Massey DC and Parkes M. Genome-wide association scanning highlights two autophagy genes, ATG16L1 and IRGM, as being significantly associated with Crohn’s disease. Autophagy2007; 3:649-51.
Fisher SA, Tremelling M, Anderson CA, et al. Genetic determinants of ulcerative colitis include the ECM1 locus and five loci implicated in Crohn’s disease. Nat. Genet.2008; 40:710-2.
Singh SB, Davis AS, Taylor GA, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41. ]
