Mimitin Antibody: Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-?B-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.
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Description
Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 ?g/mL.
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 ?g/mL.
Optimal dilutions for each application to be determined by the researcher.
Immunogen
Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin.
Isotype
IgG
Clonality/Clone
This is a polyclonal Mimitin Antibody.
Conjugate
None
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Purification
Mimitin Antibody is affinity chromatography purified via peptide column.
Host
Mimitin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Mimitin antibody can be used for detection of Mimitin by Western blot at 1 – 2 ?g/mL. Antibody can also be used for immunoflourescence starting at 20 ?g/mL. For immunofluorescence start at 20 ?g/mL.
Mimitin Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-5445 – Mimitin Peptide
Storage
Mimitin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Tsuneoka M, Teye K, Arima M, et al. A novel Myc-target gene, mimitin, that is involved in cell proliferation of esophageal squamous cell carcinoma. J. Biol. Chem.2005; 280:19977-85.
Wegrzyn P, Yarwood SJ, Fiegler N, et al. Mimitin – a novel cytokine-regulated mitochondrial protein. BMC Cell Biol.2009; 10:23.
Ogilvie I, Kennaway NG, and Shoubridge EA. A molecular chaperone for mitochondrial complex I assembly is mutated in a progressive encephalopathy. J. Clin. Invest.2005; 115:2784-92.]
