9998 in stock
Zyagen Monkey Universal Reference RNA is a high-quality standard for gene expression analysis by microarray, prepared at industrial scales to minimize lot-to-lot variation. Reference RNA is composed of highly pure intact total RNA isolated of normal healthy major tissues of male and female cynomolgus monkey adult donors including brain, heart, kidney, liver, lung, skeletal muscles, spleen, skin, stomach, intestine, colon, testis, and uterus.
RNA is isolated using modified guanidine thiocyanate techniques which allows the rapid isolation of total RNA including microRNAs. Tissues are cut in small pieces, snap frozen, grinding to powder, and homogenized in RNA denatured solution. The extracted RNA is treated with RNase-free DNase-1 to remove residual DNA, precisely quantified by NanoDrop, and stored at -80oC.
• High quality intact RNA without genomic DNA contamination
• Consistent control for standard data set comparisons
• Extracted from all male and female tissues for broad gene coverage
• Made at industrial scales to minimize lot-to-lot variation
The integrity of RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
RNA is ideal for gene expression analysis by microarray, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.
Total RNA sample is routinely provided in RNase-free water at a concentration of 1mg/ml and shipped on dry ice for overnight delivery.
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at firstname.lastname@example.org