Mouse C57 Embryo cDNA Panel, Set of 8 Embryos

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MD-104-008-C57 Category: C57 Mouse Embryo cDNA-Oligo-dT

$1,434.00

8x10reactions

9999 in stock

Product Description

Mouse C57BL/6 full length embryo cDNA panel of 8 embryos is your choice of any 8 cDNA samples (10 reactions each). The cDNA of each tissue is made of RNA extracted from freshly harvested whole embryos using classical guanidine isothiocyanate phenol: chloroform extraction method. RNA is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with oligo dT primer.

Quality Control:
The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
The synthesized cDNA is also tested as template for PCR amplification of ß-actin gene. PCR product of ß-actin was visualized on 1% agarose gel.

Applications:
cDNA is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.

Packing/shipping:
Each cDNA sample is routinely shipped on dry ice in 1.5 ml vials (10ul each) and is enough for 30 reactions (10 PCR amplifications).

MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com