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Mouse full length tissue cDNA panel is your choice of any 5 placenta cDNA samples (10 reactions each).
The full length tissue cDNA panel is made of RNA extracted from freshly harvested placentas using classical guanidine isothiocyanate-phenol:chloroform extraction method.?RNA?is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with oligo dT primer.
Quality Control:?The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
The synthesized cDNA is also tested as template for PCR amplification of ?-actin gene. PCR product of ?-actin was visualized on 1% agarose gel.
Applications:?cDNA is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.
Packing/shipping: each cDNA sample is routinely shipped on dry ice in 1.5 ml vials.
MSDS and Certificate of Analysis in PDF files:?Contact Zyagen Technical Support firstname.lastname@example.org