OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).
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Description
Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 ?g/mL.
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 ?g/mL.
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Specifications
Additional Names
OTUD4 Antibody: OTU domain containing 4, HIN1, DUBA6, HSHIN1
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4.
Specificity
This antibody is predicted to not cross-react with other members of the OTUD family.
Conjugate
None
Isotype
IgG
Clonality/Clone
This is a polyclonal OTUD4 Antibody.
Purification
OTUD4 Antibody is affinity chromatography purified via peptide column.
Host
OTUD4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 – 0.5 ?g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
OTUD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-5075 – OTUD4 Peptide
Storage
OTUD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol.2002; 10:1149-59.]
