Panel 1: Mouse CD1 Major Tissue Premade Western Blot
$1,013.00
1 Blot
9998 in stock
Product Description
Mouse CD1 major tissue Western blots is made?of high quality total proteins extracted from freshly harvested 19 major tissues of CD1 (ICR) mouse strain (Panel# 1).?Protein sample are subjected to rigorous quality control procedures before fractionation through large SDS gels.
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Blots can be used to:
Quickly assess specific protein expression across different tissues.
Determine size and relative abundance of protein in different tissues.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
The integrity of the blotted protein is tested by immunostaining of?b-actin.
Description:
The total protein?samples (75ug each )?are freshly extracted from?tissues, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE),?and blotted onto PVDF membranes. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4oC in a sealed bag with descant.
