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Panel 4: Rat Digestive Tissue Premade Northern Blot

Panel 4: Rat Digestive Tissue Premade Northern Blot

$601.00

1 Blot

9998 in stock


Product Description

Rat Digestive tissue Northern blot is made of total RNA extracted from 11 digestive tissues of Sprague Dawley rat (Panel# 4). Total RNA samples are subjected to rigorous quality control procedures?before fractionation through large denatured agarose gels.?Blots can be used with either radioactive or?nonisotopic-labeled probes to:

  • Quickly assess gene expression across rat tissues.

  • Determine size and relative abundance of transcripts in different tissues.

  • Identify alternative splice forms.

Quality control:

Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.

  • Blots are made?of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.

  • The efficiency of transfer is checked by staining gels with reversible staining .

  • The integrity of the blotted RNA is tested by beta-actin specific probe.

Description:?The freshly isolated total RNA samples (20ug each) plus one RNA marker are fractionated through large?formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blot is shipped in a sealed bag at room temperature and?is good for 1 year if properly stored at 4oC in the sealed bag.

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