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Panel 8: Mouse CD1 Tissue Custom Made Northern Blot-2

MN-CUS-2 Category:
Panel 8: Mouse CD1 Tissue Custom Made Northern Blot-2


1 Blot

9999 in stock

Product Description

Mouse CD1?Custom made Northern blot-2 is your choice of any 10 CD1 (ICR) mouse tissues listed in Panels 1 to 6. Total RNA will be extracted from the selected 10 tissues and subjected to rigorous quality control procedures?before fractionation through large denatured agarose gels.?Blots can be used with either radioactive or?nonisotopic-labeled probes to:
  • Quickly assess gene expression across mouse tissues.
  • Determine size and relative abundance of transcripts in different tissues.
  • Identify alternative splice forms.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
  • Blots are made?of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
  • The efficiency of transfer is checked by staining gels with reversible staining .
  • The integrity of the blotted RNA is tested by beta-actin specific probe.
Description:?The freshly isolated total RNA samples (20ug each) plus one RNA marker will be fractionated through large?formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blot is shipped in a sealed bag at room temperature and?is good for 1 year if properly stored at 4oC in the sealed bag.