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Product Description
APO-PM-5179
Background
PD-1 Monoclonal Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of “danger signals” such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
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Description
Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 ?g/mL.
Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 ?g/mL.
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Specifications
Additional Names
PD-1 Monoclonal Antibody: PD-1 (12A7D7), Programmed cell death 1, PDCD-1, CD279
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Clonality/Clone
This is a monoclonal PD-1 Antibody. (Clone 12A7D7)
User Note
Optimal dilutions for each application to be determined by the researcher.
Source
Mouse monoclonal PD-1 antibody was raised against an ~150 amino acid recombinant protein from near the terminus terminus of mouse PD-1.
Purification
PD-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Host
PD-1 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
A ~150 amino acid recombinant protein from near the terminus terminus of mouse PD-1.
Application
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL.
Buffer
PD-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
PD-1 monoclonal antibody can be stored at -20˚C, stable for one year.
Reference
- Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
- Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.]
Datasheet-pdf
