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Plant Tissue Custom Made Northern Blot-1
Product Description
Custom made Northern blot-1 is your choice of any 5 plant tissues of Panel# 1. Total RNA will be extracted from the selected 5 tissues and subjected to rigorous quality control procedures?before fractionation through large denatured agarose gels.?Blots can be used with either radioactive or?nonisotopic-labeled probes to:
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Quickly assess gene expression across plant tissues.
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Determine size and relative abundance of transcripts in different tissues.
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Identify alternative splice forms.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
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Blots are made?of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
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The efficiency of transfer is checked by staining gels with reversible staining .
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The integrity of the blotted RNA is tested by beta-actin specific probe.
Description:?The freshly isolated total RNA samples (20ug each) plus one RNA marker will be fractionated through large?formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blot is shipped in a sealed bag at room temperature and?is good for 1 year if properly stored at 4oC in the sealed bag.
