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Rat Mammary Gland cDNA Panel, set of any 10 Tissues

RD-414-010 Category:
Rat Mammary Gland cDNA Panel, set of any 10 Tissues


10×10 reactions

9999 in stock

Product Description

Rat ?full length cDNA mammary gland panel is your choice of any 10 samples (10 reaction each).

Full length cDNA is made of RNA extracted from freshly mammary gland using classical guanidine isothiocyanate-phenol:chloroform extraction method.?RNA?is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with oligo dT primer.

Quality Control:?The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.

The synthesized cDNA is also used as template for PCR amplification of ?-actin gene. PCR product of ?-actin was visualized on 1% agarose gel.

Applications:?cDNA is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.

Packing/shipping: each cDNA sample is routinely shipped on dry ice in 1.5 ml vials and is enough for 30 reactions (30 PCR amplifications).

MSDS and Certificate of Analysis in PDF files:?Contact Zyagen Technical Support at?