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C57 Mouse Brain Regions Total RNA

Zyagen offers high quality pure intact total RNA of many brain regions and nuclei of C57BL/6 mouse strain that might difficult to obtain because of size or anatomical complexity.

Total RNA is routinely extracted from brain tissues using the powerful classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.

RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction..

Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer.

Custom Services: Zyagen also offers custom services for isolation of total RNA and mRNA of any brain region, area, or nucleous of any mouse strain. Contact technical services for more information.

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