Zyagen Dog Universal Reference RNA is composed of highly pure intact total RNA isolated from wide variety of male and female beagle dog major tissues including brain, heart, kidney, liver, lung, skeletal muscles, spleen, skin, stomach, intestine, colon, testis, ovary and uterus. Total RNA is extracted using modified guanidine thiocyanate technique which allows the rapid isolation of total RNA including microRNAs. Tissues are cut in very small pieces, snap frozen, grinding to powder, and homogenized in RNA denatured solution for RNA purification. The extracted RNA is treated with DNase, packed at a concentration of 1mg/ml, and stored at -80oC
- High quality intact RNA without genomic DNA contamination
- Consistent control for standard data set comparisons
- Extracted from large variety of male and female dog major tissues for broad gene coverage
- Made at industrial scales to minimize lot-to-lot variation
Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.
Packing/shipping: Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at firstname.lastname@example.org
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DR-UR-100 Category: Dog Universal Reference Total RNA