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Cross-Species Western Blot

Images of different tissue proteins used for construction of Western blots and immunostaining with 2 different antibodies. Click on each image for enlargement

Multiple-species tissue Western blots are designed for screening protein expression across tissues of different species. Also for testing crossreactivity of primary polyclonal and monoclonal antibodies cross species. Blots are made of high quality total proteins which are subjected to rigorous quality control procedures. Total protein are extracted from tissues of different species in protein lysis buffer containing a cocktail of 7 mammalian protease inhibitors, fractionated through SDS-PAGE and transferred onto PVDF membranes.

 Each blot contains total protein of either brain, heart, lung, stomach, intestine, colon, liver, testis, ovary, skeletal muscle, skin, or spleen of 14 different species (human, mouse, rat, monkey, dog, cat, rabbit, guinea pig, hamster, bovine, sheep, porcine, chicken, and equine). Each lane contains 75µg of each tissue of each species

Blots can be used to:
  • screening protein expression across tissues of different species
  • Determine size and relative abundance of protein  in different tissues of different species.
  • testing crossreactivity of primary polyclonal and monoclonal antibodies cross species
  • Ready-to-use Western blots from high quality tissue lysates that are difficult to obtain because of size, high-protease tissue content, or anatomical complexity of the tissues which requires knowledge, experience, and technical skill to dissect the areas of interest freely of any surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, protein extraction, quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to labor and cost of laboratory materials, supplies and  animals (purchasing, transportation, housing, disposable of carcasses and waste materials).
  • Reusable for at least two times, just strip and use with other antibody.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
  • Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
  • The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
  • The integrity of the blotted protein is tested by immunostaining of b-actin.
The total protein samples (75ug each ) of 14 different species are freshly extracted, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes.  Each blot contains either brain, heart, lung, stomach, intestine, colon, liver, testis, ovary, skeletal muscle, skin, or spleen of 14 different species plus one prestained protein molecular weight standards. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4oC in a sealed bag with descant.
Custom Services: Zyagen also offers custom services to make ready-to-use Western blots of any set of tissues of any species specifically prepared to meet your applications, contact technical support at or at 858 546 0720  for more information.

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