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Rat Developmental Tissue WB

Rat developmental tissue Western blots are made of high quality total proteins that are extracted from several tissues of Sprague Dawley rats at different stages of life and subjected to rigorous quality control procedures before fractionation through large SDS-PAGE.
 Blots can be used to:
  • Quickly assess protein expression across rat tissues at different stages of development.
  • Determine size and relative abundance of transcripts in developmental tissues.
  • Identify alternative splice forms.
Benefits:
  • Ready-to-use Western blots from high quality tissue lysates that are difficult to obtain because of size, high-protease tissue content, or anatomical complexity of the tissues which requires knowledge, experience, and technical skill to dissect the areas of interest freely of any surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, protein extraction, quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to labor and cost of laboratory materials, supplies and  animals (purchasing, transportation, housing, disposable of carcasses and waste materials).
  • Reusable for at least two times, just strip and use with other antibody.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
  • Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
  • The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
Description:
The total protein samples  (75ug each ) are freshly extracted from tissues, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes.  Each blot contains protein samples plus one prestained protein molecular weight standards. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4oC in a sealed bag with descant.

Custom Services: Zyagen also offers custom services to make ready-to-use Western blots constructed with total proteins of any developmental tissues of any rat strains, contact technical support at zinfo@zyagen.com or at 858 546 0720  for more information.

Rat Developmental Tissue Western Blots
Lane#ts
 Brain Develop  Western Blot Heart Develop  Western Blot S Muscles Develop Western Blot Intestine Develop  Western Blot Ovary Develop  Western Blot Testis Develop  Western Blot Mammary Develop  Western Blot
Cat.#: RW-201-D Cat.#RW-801-D Cat.#: RW-102-D Cat.#RW-306-D Cat.#: RW-406-D Cat.#RW-401-D Cat.#: RW-414-D
M Protein Marker Protein Marker  Protein Marker Protein Marker Protein Marker
Protein Marker
 Protein Marker
1 E20 E20 E20 E20 2 Weeks old 2 Weeks old Non-Pregnant
2 1 Day old 1 Day old 1 Day old 1 Day old 3 Weeks old 3 Weeks old E12
3 1 Week old 1 Week old 1 Week old 1 Week old 1 Month old 1 Month old E14
4 2 Weeks old 2 Weeks old  2 Weeks old 2 Weeks old 2 Month old 2 Month old E16
5 3 Weeks old 3 Weeks old 3 Weeks old 3 Weeks old 3 Months old 3 Months old E18
 6 1 Month old 1 Month old 1 Month old 1 Month old 6 Month old 6 Month old E20
 7 2 Months old 2 Months old 2 Months old 2 Months old 12 Months old 12 Months old Day1 of Lactation
 8 3 Months old 3 Months old 3 Months old 3 Months old Day3 of Lactation
 9 6 Months old 6 Months old 6 Months old 6 Months old Day7 of Lactation
10 12 Months old 12 Months old 12 Months old 12 Months old Day1 of Involution
11 Day7 of Involution

 

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